干货分享

喜报,IF=9.56,西南大学科研团队揭示蚜虫翅型分化的分子调控机制

喜报
IF=9.56,西南大学植物保护学院王进军教授团队近日在《美国科学院院刊》(PNAS)上在线发表题为“The miR-9b microRNA mediates dimorphism and development of wing in aphids”的研究论文,发现小分子RNA介导生物胁迫因子调控蚜虫翅型分化与翅发育的分子机制,研究结果有利于寻获新的小分子RNA控制剂靶标,为蚜虫类害虫防控提供新的思路。(生物公司合作技术:IP级多克隆抗体制备)

论文链接
https://www.pnas.org/content/early/2020/03/25/1919204117
(信息来源:西南大学植物保护学院和PNAS杂志官网)
生物公司抗体定制技术服务
生物公司抗体定制平台,由多位应用设计专家和工程设计专家合力打造而成,所有技术人员均拥有多年的抗原设计和制备单/多克隆抗体的开发及生产经验。平台建立以来,已成功为客户制备了1000多种高质量抗体,其中部分抗体达到临床应用质量标准。
一站式的抗体服务、多样性的宿主系统、规模化的生产、严格的质控标准,使得生物公司的定制抗体稳定可靠,深受国内外客户的欢迎。目前生物公司抗体服务平台提供单/多克隆抗体定制及相关应用型产品的开发和批量生产,真正做到以识别內源性抗原为唯一验收标准,从根本上解决客户的痛点。
服务流程

合作案例回顾
案例一
Comparative proteomics combined with analyses of transgenic plants reveal ZmREM1.3 mediates maize resistance to southern corn rust. Plant Biotechnology Journal.
合作技术:抗体制备
抗体:ZmREM1.3兔多抗
Western blot analysis of ZmREM1.3 in transgenic maize lines 227, 229, 231 and 233 as well as the corresponding non-transgenic sib lines, with b-actin as the loading control.
案例二
In-depth Proteome of the Hypopharyngeal Glands of Honeybee Workers Reveals Highly Activated Protein and Energy Metabolism in Priming the Secretion of Royal Jelly.
合作技术:抗体制备
抗体:多种兔多抗和鼠单抗
Polyclonal rabbit antibodies against 60S ribosomal proteins RpL28, RpL26, and 40S ribosomal protein S4 (RpS4), and the monoclonal mouse antibody against major royal jelly protein 1 (MRJP1) were developed (Genecreate Biological Engineering, Wuhan, China).
案例三
Divergent molecular evolution in glutathione S-transferase conferring malathionresistance in the oriental fruit fly, Bactrocera dorsalis (Hendel).
合作技术:抗体制备
抗体:制备MR、MS特异性抗体
案例四
CRD1, an Xpo1 domain protein, regulates miRNA accumulation and crown root development in rice, plant journal.
合作技术:抗体制备
抗体:抗CRD 1多克隆抗体
(c) Immunostaining of CRD1-GFP (red fluorescence) in cross-sections of root tips of HJ2 (upper panel) and CRD1-GFP lines (lower panel). (d) Validation of CRD1 antibody (Anti-CRD1) using immunoblot analysis. (e) Subcellular localization of CRD1 in HJ2 analyzed by immunoblot analysis.
案例五
Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array,Analytica Chimica Acta.
合作技术:抗体制备
抗体:CHIL-4和ChIFN-4的单抗、纯化的重组CHIL-4和ChIFN-1抗原
For the first incubation, the surface capture of the cytokines by their respective primary antibodies anti-ChIL-4 and anti-ChIFN-γ, the subsequent CL intensity value increased with increasing incubation time up to a maximum at 30 min (Fig.2a). For the second step, formation of the sandwich via the attachment of the Ab2-AuNP-HRP probe to the exposed Ab1-cytokine-immunocomplex, the maximum CL value was similarly reached with only a 25 min incubation (Fig.2b).

(0)

本文由 生物知识学习 作者:来源互联网 发表,转载请注明来源!

热评文章

发表评论